The expression of each gene of interest was profiled using a LightCycler 480 (Roche Applied Science, Mannheim, Germany) using the LightCycler 480 SYBR Green master mix (Roche Applied Science)
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Mobile phase A (MPA) consisted of 60/40 acetonitrile/water with 10 mM ammonium formate and 0.1% formic acid, while mobile phase B (MPB) was composed of 89.1/9.9/0.99 isopropanol/acetonitrile/water with the same additives
Compounding pharmacies can compound medications without clinically significant reasons during shortages, but they may still provide compounded formulations with valid medical justifications, such as excipient-free formulations, specific concentration adjustments, or alternate delivery forms
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J Anim Sci 94:26032613 Wu ZL, Hou YQ, Dai ZL et al (2019) Metabolism, nutrition and redox signaling of hydroxyproline
