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Most lyophilization cycles take from several to many hours, sometimes even days, depending on freezing step , primary drying , and secondary drying duration

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Tissue peroxidase was inactivated by incubating in 3% hydrogen peroxide in TBS for 30 min

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Data analysis of in vitro pharmacology experiments Nonlinear regression and Schild plot analyses were performed with GraphPad Prism 6 (GraphPad Software, San Diego)