For assessing the change in relative IBA1 or GFAP abundance at different doses of LPS, a linear model was constructed for each protein (IBA1 or GFAP) and each brain region (hippocampus or entire brain) using the protein percentage as dependent variable and treatment (veh-PBS, veh-LPS-0.05, veh-LPS-0.1, veh-LPS-0.5, and veh-LPS-1.0) and time (2d and 11d after the last LPS injection) as independent variables with interactions effects
Second, the mechanistic stratification across nitric-oxide, cytoskeletal, and copper-mediated ECM pathways gives research designs clean tool separation
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