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Before taking PT-141, make sure to tell your doctor if youre taking any of the following medications

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Proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Thermo Fisher Scientific, #88518, 0.45 m) using a wet-tank blotting system (Bio-Rad) with pre-cooled transfer buffer (25 mM Tris, 0.2 M glycine, 10% methanol) at 100 V for 30 min