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The expression of each gene of interest was profiled using a LightCycler 480 (Roche Applied Science, Mannheim, Germany) using the LightCycler 480 SYBR Green master mix (Roche Applied Science)

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Inhibition of DPP4 activity by synthetic inhibitors, such as those used to treat type 2 diabetes, results in suppression of T cell proliferation in vitro and a decrease in antibody production in vivo

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SCFAs acetate, propionate, and butyrate were the main pathways that inhibited PPAR expression and activated the AMP-activated protein kinase (AMPK) pathway in this experiment (64)