These homogenates were then washed with 10 mL PBS in scintillation vials (at least 5 buffer changes over two days)
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The preprocessing pipeline, developed from our previously described pipeline 101,106,107 , was adapted with software substitutions
Notably, the antimicrobial activity of polyphenols can be influenced by the extraction procedure and the solvent used (339, 353)
This initial dose allows the body to adapt before progressing to higher doses