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TrxR1 Activity Assays TrxR1 activity was assessed using 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB) (D8130-5G, Sigma) also known as Ellmans reagent to detect the rate and level of reductive activity from TrxR1

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Measurement of cell viability, apoptosis and glutathione reductase activity HepG2 cells (purchased from Shanghai Institutes for Biological Sciences, Shanghai, China) were cultured in type B medium containing 12.76 nmol/L riboflavin before being transferred into the media with different riboflavin concentrations (0.76, 3.76, 12.76, 24.76, 48.76 nmol/L)

Menka Gupta, M.D., is a physician and functional medicine certified practitioner (IFMCP) in Singapore

doi: 10.1016/j.cell.2018.08.047 190 ZmoraNZilberman-SchapiraGSuezJMorUDori-BachashMBashiardesSet al