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Sample preparations for plasma, urine, hair, and tissue All samples were processed with a standard operating procedure.150 L aliquots of thawed plasma or urine were mixed with three internal standards (IS) [20 L of d4-alanine (Sigma, USA, 10 mM), d5-phenylalanine (Sigma, USA, 10 mM), and d5-tryptophan (Sigma, USA, 10 mM)]

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The specific concentration, exposure time, and experimental protocols are determined by the researcher based on the study design, existing literature, and institutional guidelines

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