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When the flow rate was changed, it was found that a flow rate of 1 mL min -1 gave the best results for getting resolved peaks with the least amount of solvent use and run time

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Traditional nanomaterials exert antibacterial effects by releasing metal ions such as Ag + , Cu 2+ , and Zn 2+ , which disrupt bacterial cell membranes and interfere with enzymatic activities (Xiu et al., 2011