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Challenges and Future Directions of GLP-1 'Central Program' Intervention Current challenge: Individual differences and long-term safety Although GLP-1 injections have shown excellent efficacy in clinical trials, their "central plan" interventions still face challenges: Individual metabolic differences There are significant differences in the response of different patients to GLP-1RA, which are related to factors such as gene polymorphism, gut microbiota composition, and metabolic flexibility

10.1056/NEJMoa2307563 57 LiuJ.WeiL.WangZ.SongS.LinZ.ZhuJ.et al (2020a)

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Purification Protocol Purification of soluble GST-tagged proteins under native conditions