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NADPH levels were assessed by measuring the fluorescence ratio at 407 and 482 nm (Olympus IX83 microscope), with quantification performed in ImageJ, as described previously 29

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However, insulin sensitivity and glucose tolerance were maintained, likely owing to an increased conversion of saturated fatty acids (known to cause insulin resistance) to monounsaturated fatty acids mediated by stearoyl-CoA desaturase-1 (SCD1) [52]