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Western immunoblotting Protein samples were denatured with 2 x SDS buffer, separated on NuPAGE 412% Bis-Tris Gel (NP0335

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The NLS is composed of two clusters of basic amino acids separated by ten residues (617-RKCYEAGMTLGARKLKK-634), a motif that is highly conserved with that of the GR, MR and PR steroid hormone receptors

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