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The Zymo-Spin VI with Zymo Maxi Filter assembly can be used with vacuum manifolds for the purification of DNA or RNA
including PCR amplification
Input volumes can range up to 10 mL of plasma
The methylated DNA standard is purified HCT116 DKO DNA that has been enzymatically methylated at CpG sites
The included Reaction Buffer (4X) contains dNTPs
RRHP 5-hmC Library Prep Kit contact-for-pricing The Zymo-Spin VI with ZymoThe RRHP 5 hmC Library Prep Kit is an all inclusive solution for analysis of genome wide 5 hydroxymethylcytosine (5 hmC) positions at single base resolution. The Reduced Representation Hydroxymethylation Profiling (RRHP) method is based on blocking MspI digestion by glucosylating 5 hmC within MspI recognition sites. Fragments lacking glucosylated 5 hmC at the adapter ligation junction will be cleaved and not amplified by PCR. Therefore, only fragments
