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The reaction was quenched after incubation for 8 h at 30 C by addition of equivalent volume (150 l) of stopping solution (10% (w/v) FeCl 3 and 3.3% (w/v) trichloroacetic acid dissolved in 0.7 M HCl)

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Inside that outer ring sits the nucleus pulposus , a gelatinous core rich in type II collagen, elastin fibers, and a hydrated proteoglycan gel that gives the disc its shock-absorbing properties

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