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The classic mechanism of acetaminophen hepatotoxicity is that its reactive metabolite, N-acetyl-p-benzoquinoneimine (NAPQI), depletes GSH and then covalently binds to proteins

Key takeaway Glutathione synthesis is a nutrient-dependent, stepwise process

Some bacteria use one bifunctional enzyme, called GshF, to carry out both steps

Bacteriostatic water is the correct solvent

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These findings underline the important role of CYPOR as an electron donor for the reduction of NFT, but do not exclude the involvement of other reductases in NFT reduction