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The cell suspensions were washed twice with ice-cold PBS before staining using an Annexin V/PI staining kit (Alexa Fluor 488 annexin V/Dead Cell Apoptosis Kit)

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This response encourages fibroblasts within the dermis to become more active, supporting the production of collagen fibers that contribute to skin firmness and structure

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Under physiological conditions, mitochondrial glutathione preserves the redox environment required for oxidative phosphorylation and protects cardiolipin-rich inner membranes from peroxidative damage